FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30979.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30979.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22169888
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG247550.016.6048365
CTTACAC141350.014.82838253
CTACACT149250.014.4756454
TACACTG196700.014.2633345
GTGTAGG107350.014.2074781
CTATACT162600.014.1789014
CCTACAC105900.013.9709513
GTATAGA166700.013.5495391
TCTATAC164700.013.4747153
CTAGGAC95600.013.4278713
GTGTAAG146200.013.3630211
ATACTGT324550.013.1338646
CCTATAC86100.013.0563063
GTCCTAG104750.013.0347761
GTGTTAG174250.012.9624571
GTATAGG90250.012.95622351
TAGACTG156200.012.8562165
GTCTTAC113950.012.6834821
GTGTAGC95150.012.3653371
CTGTCGC74050.012.3348119
Produced by FastQC (version 0.11.5)