FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30976.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30976.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28934856
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG364230.12587932008370803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG27700.017.54219145
TACACTG259200.015.3547575
TATACTG357350.014.7687325
GCCGACC64250.014.674355145
GTGTAGG135150.014.2431541
GTATAGG113600.014.1315661
CTACACT187650.013.8704264
GTATTAG205950.013.6145041
CTATACT217900.013.47544
GTATAGA211450.013.2947321
GTGTTAG224150.012.995171
GTGTAAG188400.012.9163631
TACACAG320850.012.7433225
CCTACAC131550.012.735633
TCTATAC210000.012.6058713
GTCCTAG135900.012.5610151
TCTACAC188450.012.3925033
GTCTAAG137500.012.3620221
CTTAGAC136900.012.3438823
TACACCG33850.012.2073615
Produced by FastQC (version 0.11.5)