FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30893.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30893.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33111353
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCC76900.021.596535145
GCCGACC85550.019.497671145
CGACGAA76450.019.44694145
CCGTATA62300.018.392635145
TATACTG367200.016.1106175
CTACACT204200.015.3740744
TACACTG271250.015.0741925
GTATAGA232950.014.8737561
TACACCG42700.014.771215
CTGCCGC365950.014.724548145
CTATACT228450.014.4403284
GTATAGG121250.014.1981451
GTATTAG226050.014.0424191
TGACGCG40400.013.822425145
TCTATAC225650.013.5318483
GTGTAAG204300.013.5107741
CCTACAC145650.013.3454933
TCTACAC199000.013.3394633
GTCCTAG142900.013.2670161
CGGTGGG133400.013.156321145
Produced by FastQC (version 0.11.5)