FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30890.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30890.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25825855
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288990.11189948987168091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG23450.019.793144145
GCCGACC48900.016.462349145
TATACTG288300.015.2382455
GTGTAGG118000.015.1430261
CGACGAA28000.015.022687145
GTATAGA186500.013.82641
CTATACT193850.013.7262734
TACACTG229750.013.1894815
CTTACAC166700.012.7914483
CCTACAC116250.012.789953
GTATTAG188350.012.5722081
TCTATAC183850.012.5450223
GTCTAAG125100.012.4836251
TCTACAC164650.012.4661593
GTGTAAG171800.012.3880821
CTACACT169250.012.3800254
GTCCTAC79050.012.312961
TACACAG293100.012.2679735
CGCCGTT25650.012.157906145
GTATAGG104550.012.0888441
Produced by FastQC (version 0.11.5)