FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30849.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30849.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25525794
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG312100.015.8880055
GCCGACC49000.015.541335145
CTATACT198300.014.7338634
TACACTG201800.014.549265
CTACACT159900.013.5567934
TCTATAC188850.013.4413193
GTATAGG97450.012.9696861
ACGATCC53350.012.914699145
GTATTAG202850.012.8552791
CTAGTAC81600.012.4431043
CGACGAA43800.012.418876145
CCTATAC101500.012.3614933
CCTACAC112350.012.2651073
CTGCCGC205750.012.196392145
TACACAG257950.012.1691275
GTGTAGG114450.012.0586821
GTAGGAC74600.012.0551813
CCGACGC53800.011.997833145
GTATAGA191050.011.9003391
TACACCG36000.011.8810975
Produced by FastQC (version 0.11.5)