FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30844.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30844.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25911395
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289010.11153780026123641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG219650.014.7856945
TATACCG86350.014.1879385
TACACTG148400.014.0686855
CTATACT189250.013.4077654
TACACCG112300.013.2978785
GTATTAG206750.013.1738011
CTAGACT82150.013.1493084
CTAGGAC65850.012.4452753
GTATAAG209150.012.3975471
GTATAGG132250.012.35697651
GTCCTAG65300.012.2350261
GTATAGA210850.012.2286961
GTCTTAG139950.012.1960821
TTATACT327500.012.1309134
TACACAG182600.012.06895
GTGTAGG132900.011.8593291
GTCCTAC98750.011.8417031
TCTAGAC85950.011.8131023
TGGACCG70750.011.7832835
TATACAG232000.011.7175875
Produced by FastQC (version 0.11.5)