FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30830.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30830.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27809040
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390730.14050467042371834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG32050.018.10241145
GCCGACC62900.017.755976145
TATACTG297250.015.2185165
CCGTATA43600.015.136635145
TACACTG234300.015.0374295
CTGCCGC231800.014.923798145
CTATACT194100.014.2311344
CTACACT172300.014.2223554
TATACCG29550.013.7385265
GTGTAGG121950.013.6990241
GTATAGG108000.013.4508541
CCTACAC123500.013.4479543
GTATAGA193600.013.43141
GCTCGGA42150.013.4149876
CGACGAA56300.013.396748145
TCTATAC189650.013.3080413
GTATTAG195100.013.0675311
GGGCTCG47250.013.0423914
CTAGGAC120200.012.8517673
GTGTTAG200100.012.7047051
Produced by FastQC (version 0.11.5)