FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30786.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30786.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26513779
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305080.11506469900047067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC138500.023.616339145
GCCGACC60100.015.928881145
GACGACA59000.014.996628145
TACACTG228500.014.8799965
TATACTG313750.014.7187835
CTACACT169150.013.8872764
GTATTAG187500.013.5982211
GTATAGA195650.013.5886861
CTGCCGC254500.013.507542145
CCTACAC119250.013.5021883
CTATACT197750.013.491974
GTATAGG104050.013.4738421
TCTATAC194550.013.3090333
TCTACAC172600.012.90051653
TACACAG279800.012.540475
GTGTAAG174000.012.2319191
GTACTAG80500.012.0916391
GTAGGAC79700.012.0122713
CCTAGAC114050.011.955623
TAGACTG181250.011.8794045
Produced by FastQC (version 0.11.5)