FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30782.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30782.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28227174
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347660.12316500404893525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG88850.015.0947055
TATACTG237800.014.5417555
TACACTG161250.014.0270145
TACACCG129400.013.8940125
CTACACT147800.013.2443194
CTATACT195900.013.2121694
GTATAGA225550.013.1724451
TACACAG187550.012.8717475
GTCCTAG79500.012.7922471
GTATAAG208750.012.63182451
GTGTAGG153350.012.5056441
TATACAG242500.012.4662155
CTAGACT91000.012.42863754
GTATAGG138050.012.2604231
TCTATAC220200.012.1865543
GTGTAAG153900.012.177751
GTATTAG223600.012.1502651
TCTACAC199800.012.1240443
TTATACT335200.012.0040914
TAGACAG186750.011.8399415
Produced by FastQC (version 0.11.5)