FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30782.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30782.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25571165
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316200.12365490582849861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG266200.015.4142035
CCGACGC121300.015.0670805145
CTATACT176100.014.6565364
TACACTG189850.014.1287525
TACACCG66150.013.4799395
TATACCG46050.013.38141255
TCTATAC184650.013.1577193
CTACACT151350.013.0773864
GTGTAGG123100.013.0392541
CTTACAC147600.012.8736013
GTATAAG172550.012.29097751
GTATTAG198200.012.2027891
TACACAG228050.011.9528235
GTATAGG105200.011.9439751
CTAGGAC98850.011.8122963
GTATAGA186250.011.7378551
CCTACAC119900.011.7345843
TCTACAC165650.011.7335243
GTATACG46650.011.6769151
GTGTAAG151150.011.6285541
Produced by FastQC (version 0.11.5)