FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28157409
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2045290.7263772032433808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG150050.063.9143681
GCTCGGA52650.041.443066
GGGCTCG54900.039.744574
CGGAGAT55050.039.5005269
TCGGAGA56750.038.189538
CTCGGAG57050.037.9887737
GGCTCGG60400.036.245475
CGTGGGC78450.028.21821
GTGGGCT122050.018.4174822
TGGGCTC140750.016.4829963
TATACTG160900.014.7324655
CTAGGAC54500.013.8347543
TACACTG118700.013.2523445
TACACCG30700.013.2231025
TAGGACA83700.012.7313824
CTACACT101250.012.5292974
TCTCCGC100400.012.489748145
GTGTAAG86950.012.4785861
GTGGGGG900100.011.9469412
CTAGTAC46300.011.7439683
Produced by FastQC (version 0.11.5)