FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_Neg.7.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_Neg.7.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAATCAAAATGCCTTCTTTCAAGCAAAAAAAAGTCATGTATTTCTTT10.78125No Hit
GTGAGTGGGTGGTTCCTCAGATTATGAGGTAATGGATATGAAAGGATTTT10.78125No Hit
TCAGTATATTTTGCAATCACTATCTAAAACGAAACCATCTTAAATTCAAA10.78125No Hit
GTTCTGGAGGCAGAGGGAAAAAGGAAAGAGAGCTGGGTGGGACTGGAATA10.78125No Hit
CTTCCATTCCACTCCAGTCCATTCCATTCCTGTCCATTCCATTCCAGTCC10.78125No Hit
CCACAGAGCCAGCAGGAGTTGGGAACAGGCAGGAGCCCCATCCCCTTCCA10.78125No Hit
GAAATCTTGGTATTTAGCCATGTAACTGCTAAAAATATGTTGCACAAGTT10.78125No Hit
AGAGTGGCCACATCAGGCTTTCCTTGGAGTCCCTTTGCATTCTCTCGAAG10.78125No Hit
ACTCTGTGACTTGAATGCAAACATCACAAAGAAGTTACTGCGAATGCTGC10.78125No Hit
ATTTGGGATCACCAGAACTCCCAACACGCAACTACTGCACTCTCCTTCTG10.78125No Hit
TTGCTACTTCTCTAATTGCCATTTCTGTTATAGTATTCCTGTCTCTTATA10.78125No Hit
GAGCAAGACCCTGCCTCCAAAGAAGAAAAGAAAAGAAAGAAAAAGGAAAG10.78125No Hit
TCCGAGTGTTCTCATTATTCAATTCCCACCTATGAGTGAGAACATGCGGT10.78125No Hit
GTCTTATGGTGAAAACGGAAATTACTTCACACAAAAAGTAGACAGAAGCG10.78125No Hit
GGTCAGGAGTTCGAGCCTAGACTGGCCAACATGGTGAAACCCCATCTCAA10.78125No Hit
GGGTTGCTTAGTCCTGATATACACAATCAAGGAAGATGCAAAATTTATTT10.78125No Hit
GGCTAACCCTAAGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGT10.78125No Hit
CCATCATTTTCTTTGCTTCCTTTTAAAGCAAAATTTTCCCCAAATATTTT10.78125No Hit
CTCTTAAGTTGCTCGTGTGGTTGTAAATTGGAGATAACTTGCATGATTGT10.78125No Hit
GGGCGGGGGTGGCGAGAGAGAGGAGATGAGTAGCAGCGCTTTGGGGGTGC10.78125No Hit
GCCGTGTACTGTTGGTGCAGCGTTTTGGTCAGAATTTCATCCGCGTGGTT10.78125No Hit
GCTCTAAATGGAGAATAGGATGTAATGTGCATGGTTTCTTAGTTTTATTT10.78125No Hit
CCTCAAAGCGCTGCGGTTACAGGCATGAGCCACCGCGCCTAGCCCCACCC10.78125No Hit
GGGAGAGGCTGATGGGGAAGCAGTGTGGGAACACTGAGGCCCAGCCACGA10.78125No Hit
GGCGATCCATGCACCTAAAGGGGCACTTAGTTTTGTGCGTTTGTTCTTTT10.78125No Hit
ATACAAGGCCATCTCAGAGAGCGTGGGCTTATGCTGGGTCTGGAGGTAGA10.78125No Hit
GTATAATCAACATAAAATATAAAACTACACAAAGTATCTATTATATACTG10.78125No Hit
CCCCTCCACCCCCCATTCATGTGAAAAGGGTGACCAAACAGGGCAAAAGG10.78125No Hit
CTATGCAACTCTTCTGAGGATCAAAGTTTCACTTTAAGCCAGTTGGAGCT10.78125No Hit
AGACTAAGAAGAGTTCTCAGTAACTTTTTTGTGTTGTGTGTATTCAACTC10.78125No Hit
AGGGGAAGTTATCTTGACTATCAACTTGTTTCCACTTAATCATTGGTCAA10.78125No Hit
CTATATGAGTGAGTATATTTAGCTGGTTCTATAATTTTATTTGATTGTCT10.78125No Hit
ACCTACAGTTGGGCAAAATCCTCTGAGACAAAGCCTTATTTGTGTAAATA10.78125No Hit
ATTAGGAGCAATATCACCTTGGGGGTGGGCACCTTCTGTGATATTGGTAG10.78125No Hit
GTGAGGGACTCAATGAATTTTTCGATTCAACTCTCTATATTTTTTTAAAA10.78125No Hit
GTGTTCCCAATTGTGTTATAGCACTCATGGCAGACATACAGAATCCCATC10.78125No Hit
AAGTCTATCTGGGGGAGGACTTCTAAAAAGGAGTTATAATAGATTTGATT10.78125No Hit
GACGGATATTATGAAATTGATTTTGAGAAGTTTGAAGAGTTTGCGAAAGA10.78125No Hit
GCGTGTCACAGTATCAAAAAGCACAGTCCGTGAGCTCATCTCGGCTCCAA10.78125No Hit
TAATAGTACTTAAAGTGTAAACTAATAACATCATGCTCTAAAAATAATCA10.78125No Hit
CATGCACTCACCCCCCAGATGTTGGGGTGGGCGTCATTTTTTAAAAATTG10.78125No Hit
ATCATCAGGTACCATTTTGACACAAGATCTAATCCAATCTACCGGGATAA10.78125No Hit
GCCGGGTGGCTGCATTTTCAGTTTGTCATTAGTACAGACGGCTACATGGG10.78125No Hit
GATGTGTCCTGTGTCTCAGAAGACTGAAGGCTGCCACTAAGTTTGCAGAA10.78125No Hit
GCCACGTTCTTTGCTACAGCAAAACAAAAGTAACCCTGGCTCCTGTACCC10.78125No Hit
AGCCTGCCGTGTCTGTCATTGTTTGTTTTTAAATGTGTAGTAGTAGGAAG10.78125No Hit
GTTATTTGGTGTATAGTCAGGGTTTTCTAGAAGGACAGAACGAATAGGAT10.78125No Hit
ATTAAGGAGTCATTTTGCATTTTTTCCATTGAAATATCTAATCAAGATTT10.78125No Hit
CCTAATATACTTACATTGTTTATTATTTAAAGATAATATTCTAGGTAACA10.78125No Hit
GTTATATATAGTACATCAGAGGGTGATGATTAGTGCTTTGGAGAGTAATG10.78125No Hit
ATGTGAGGTTGTGGAAGTTAATTAATTTCCTGGAGTTTCCTGTTACTCAT10.78125No Hit
CTCATAAGTGGGAGCTAAGCTATGAGGACACGAAGGCATAATAATTAAAA10.78125No Hit
AGAAATCACAGGTTATGCGATAATTTTGTGAGATCTACCTTTTTGGGAAA10.78125No Hit
TTATAATGAGGTTCTGAAATCAAGTTATACCAATATGTTCACCCATAATT10.78125No Hit
CGGGTCTTGGTCCTAGACTGCGGGGGAATGAGTGGTACCCTGAAGTTTGA10.78125No Hit
AGGTAGATGAGGAAGCTCCCAAGGAAATCTTATGGAGTGCTGGAAATTAC10.78125No Hit
ACATAATTTTTAAGCAGTGTATTAATGCATAAAGATAATTTGTAATTTAG10.78125No Hit
CGTCATATTTTTCCTCTGGTTCTCATGCTCCTGGCCCCAGAATAAAGAAA10.78125No Hit
GTGTCTTTGCAGCCTTTCCATGGCACTTAGTGATGAATGAGTCTAGTTTC10.78125No Hit
AATCAAGAGAAGACACGTCTAGCAGAACATTTATTGAACGTTTTTTAATG10.78125No Hit
GCATACAATAGTTTGAATAATGATTATTCAACTTTAAATAGGATATCAAA10.78125No Hit
GTCCCATCCACCATCCAAGGGCTGAGGAGTGCGGGCACACTGCATCGGAC10.78125No Hit
GTCTAAAACATCAAAATTGGGGATACATGTATTACTTTTAAATACTGAGG10.78125No Hit
CATGTATTTCGTCATAATACGGAGAAACCCATAACTGTTAATGGAAGTGA10.78125No Hit
GTCCCTGGGCGTGCGCAAGGGTGACCGCGTGGTCATCTACCTGCCCGTCA10.78125No Hit
AACAAGTTTCACAGAATGCTTCTTTCTAGCTTGTAGGGGAAGATATTCCC10.78125No Hit
CTGTCTGGTTTTATAGATTTCAAAAAAGGTTCAGAGCTACTAGAGCGAAA10.78125No Hit
GGTTGCTCCTAAGTTCTGTGGACAAAGTGAGGAAAGAAAATGATGAACTC10.78125No Hit
CTAATATATATACGATTAGATATACATATTATATATAAATCTCATTACAT10.78125No Hit
GAATAAAAAAAGGAAGAATGGAGGAAGGCAGAAAGAAAGAGTGTAATGGT10.78125No Hit
CTCACTTGCTTTGGTGACTTTTATAATTTGGGCAATATACTAACAAGGAT10.78125No Hit
ATAATATCCTCAGAGAAGTGAGAAATTTCTTCATTAACCAAGATTGGATG10.78125No Hit
GTTACATGTCTTTTGCTTTTGAATCTTATCTGCATCCAAATAAGTAAATT10.78125No Hit
AAATAAAATTGAATTTTTGAAATTAAGTATGCATATATTTTATGGGCAAT10.78125No Hit
ATTTTGGCTTTTGTTGCCATTGCTTTTGGTGTTTTAGACATGAAGTCCTT10.78125No Hit
TTGCGAAACTGACGGGGTTCGCGGCGGTACGACCACACAAAAATGGCTCC10.78125No Hit
GCGGCCGCCGCAGCCTACGGATAAGCTAGAATTGTTAATTGTTATAGCCT10.78125No Hit
GAAGTGGGCCCTGTCCAGACATTAGCTTTTGGGCTTCCCACACTCCAGAA10.78125No Hit
ATATTGTGCTATTTAATATGTAGTTAAGAATGCTGTGTCTCAGTCCATTT10.78125No Hit
TGCAAGCATGGCTCAACATATACAAATCAATCAATGTGATACATCATATC10.78125No Hit
CAAGACATGAGTTTACAAATGTAACAACACTTAAAATATAACCATGAATC10.78125No Hit
CAAAAGCGATCCAGAAATTTAGGAACCACTCCTCTTATGATTAAGTTGTC10.78125No Hit
TATAAACACTGTAAACAGGCCATTGCTTAATAAGGTAGATTTTCTGACAA10.78125No Hit
TACTTACTCAAAACAACAAAAAACAAAAAAACACACACCACGATCCCAAG10.78125No Hit
CTATTTTTGTTTCTTTTGATATAGCATCTATAATTTTATAATTAGTATTT10.78125No Hit
CCAGTAACTTGAAACAGCAGAAATTTATTTTTTTCACAGTCCTGAAGGCT10.78125No Hit
GTATTTTCGTTCGTTAACATTATTACTAAACTTAAAAGTAAATCACACAA10.78125No Hit
GTCTGTTTCATCTTCAGTATTACAAAGCGGAATTTCTGAAGAGAAATGTG10.78125No Hit
AAAAACACACAACACAAGCAAGATACTAATAAAACAACTGTCTTCCACAT10.78125No Hit
TTTCAGCATCTTTTTAGATTATCATCTTTGTTTCCGCATTGTTTCTGTTG10.78125No Hit
GTGCGCCCACACTCCTCAGCCCTTGGGTGGTCGATGGGACTAGGTGCTGT10.78125No Hit
ATATGGCTCTGTTTCAAAAAACAATTACACAAGAATCTACAAGGGATTTT10.78125No Hit
CTTGAAAACTGATGAGTTAGGACAAGAAACTGTGTGCCTGAGAATAGTTT10.78125No Hit
GTTTAACTCAGGATCTTCAAAATAGATGCCAAAGTCCAATTACTTTTTAC10.78125No Hit
AACATTAACAATTTAGAGTAAGATAATAAAATACATATGCATACAAATGC10.78125No Hit
GATACGGTATAGGCACTTAGAAAAAAAGAGTAAGATCTTTACAAAAACCT10.78125No Hit
CTATAACTCTCTAATTTATTAGAAAATCTCCTTCTGTCATTCCCAGAAGT10.78125No Hit
GGCTAGAAGGAAGCTGCACAATATAGATCAATGTAGAAGGAAAAAAAAAT10.78125No Hit
ATTGTCTTCAATAAGATTTTCATCTTCTGAATAGAGCTTGTCTAAAATCC10.78125No Hit
CTCAAACACATATTATAAATATTATTTAAAATTTATTGATACCTCATAAG10.78125No Hit
GCCCGGGTGCGGCCGGCCCCCCTGCAGCGAGCCTGGTGACGGCCCCCAGT10.78125No Hit
TAGAAAAAAACAAATAATATAAAAAAATAAGAGTATTTAAATAACCTACA10.78125No Hit
GTTTCACTCTTGTAGCCTAGGCTGGAGTGCAGTGGCATGATCTCGGCTGA10.78125No Hit
GTGTCTAGCATAGCGTCCAGCACAGGCAGACTATCAAGCCTGTTTTGATT10.78125No Hit
GATAAATAGATTAATATTATTTCCATTATTTTCTATCATATAATACGTGA10.78125No Hit
CCATCCTACTTCTCCAGACCCAAAACTCCCAATTCTCCTGGACACATTAT10.78125No Hit
GTCTTGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCACAATCTCGGCTCAC10.78125No Hit
GGATCTTAAACAAAAAAAAAAAAAAAAAAAAAACAACAAACAAAAACATA10.78125No Hit
GGTCTGGAGATCGAGACCTTGCGGGGAAAGCCTGTGAAGGTCCATCTCTA10.78125No Hit
GCCCTATCAACTGTAAAGAGAGCCACACTCATCAGGATATCGAACAGCAA10.78125No Hit
GTGTAGGGGTGTATGTGTGTATGAGTGTAGGTATGTATGTGTGTAGGTGT10.78125No Hit
GTGGGGGGCTGCGCTGGGGGTCGCGGCGGGCTCGGCCGGCCGCCTGCTGC10.78125No Hit
GAATAGAGGGGAAAAGCCAAGAATTCTGGGTTTGATCCACCTGGCTTTGA10.78125No Hit
AAACAAAACAAACAAACAAAAAGTGATTACCTCCAAAAGCAAATCCACAC10.78125No Hit
TCGTTAGGATTATGATTTTAAATTATAACTGCTTACTGTGATTTGTGTTT10.78125No Hit
ATATTTATGTCTCCTCGTATGTCTCTTATATGCCCATATAATATTCTGTG10.78125No Hit
GTTTGAGTTACAACCGTTGGATCTGCCTTCCTTTTTCAGTATTTCAGCAG10.78125No Hit
AATTCATGCCGTCTGCCACCATTTGAGTCTTATTTGTCTATGTTTAATTA10.78125No Hit
GGCTACATCTATGGTATCTGGTATTTGATCCATCGCCAATTGCTCATCCC10.78125No Hit
AGTATAAGAAGATATTCAATGGTAAATTTACCATTTTATAATTAACTGCA10.78125No Hit
ATACATGATCACGACTCTGGTGTTGAAGATGAAGATTTTTCTCCAAGACC10.78125No Hit
CTTTACAGGAGACGCCTGCTGTGAAGATGCATTGTAAATTGTGAAGCGTT10.78125No Hit
GTGGGATGGGGAGCTAAAGGGGATGGAGTGGGAAGATAATCTTCTCTTGG10.78125No Hit
GTCGAGTTGTTTTAGATGGATTACACCTGTTTCTGAACAAATCAAATACA10.78125No Hit
AGCTCGGACTCCAGAACTTCGACACCTGCAGTATAGATTTCATGATATTC10.78125No Hit
CCTTACCACCATATGTCGAGGCTGGATTTAAGTTGGGGATGAGGATGGAA10.78125No Hit
CACCAATGTAATTGTCTTTAACTTTAACCACATCCTGGCAAACCCTTGCT10.78125No Hit
TAATAATCCTTTGGCTATATACCCAGTAATGGAATTGCTGGGTCGAATGG10.78125No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers