FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_Neg.7.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_Neg.7.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5855
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC60.10247651579846286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG100.006912936145.05
GGCGTAT100.006912936145.01
TACGGCG100.006912936145.07
GTATACG100.006912936145.04
ATACGGC100.006912936145.06
AAGGCTA100.006912936145.0145
CGTATAC100.006912936145.03
ATGTTGT151.1689179E-4145.06
GCGTATA100.006912936145.02
TGGCTTT100.006912936145.06
TATACAC353.1591182E-582.857143
CTTATAC353.1591182E-582.857141
ATACACA451.0860458E-680.555564
CACATCT406.1254534E-572.57
CATCTCC406.1254534E-572.59
ACATCTC406.1254534E-572.58
TTATACA451.0977761E-464.444442
TACACAT451.0977761E-464.444445
ACACATC451.0977761E-464.444446
GCCCACG300.001459948124.16666815-19
Produced by FastQC (version 0.11.5)