FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_Neg.7.16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_Neg.7.16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5254
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT390.7422915873620098No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGGCGT90.17129805862200229No Hit
CTATGGGACTTGTTATTCCTTAAGCAATTTGGGGCATTGGTCCTCCTCCA60.11419870574800152No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT60.11419870574800152No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGG60.11419870574800152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGC100.006892607145.03
CACATCT352.3953726E-7103.571437
ACATCTC352.3953726E-7103.571438
TACACAT352.3953726E-7103.571435
TATACAC405.307684E-790.6253
CATCTCC405.307684E-790.6259
TTATACA405.307684E-790.6252
ACACATC405.307684E-790.6256
ATACACA405.307684E-790.6254
CTTATAC502.0022871E-672.51
GGACTCC200.00596876729.025-29
ACTCCTA200.00596876729.030-34
CGGACTC200.00596876729.025-29
GACTCCT200.00596876729.030-34
GAAAAAA350.0035660120.71428760-64
CCCACGA350.0035660120.71428715-19
CGAGACG350.0035660120.71428720-24
AGCCCAC400.007717885518.12515-19
ATCTCCG400.007717885518.12510-14
CCACGAG400.007717885518.12520-24
Produced by FastQC (version 0.11.5)