FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_Neg.7.10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_Neg.7.10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCAA100.0068621617145.02
AGGTGTA100.0068621617145.07
TAAGGTG100.0068621617145.05
GGTGTAA100.0068621617145.08
CACATCT400.005672478654.3757
CATCTCC400.005672478654.3759
TATACAC450.00903847848.3333323
ACATCTC450.00903847848.3333328
TTATACA450.00903847848.3333322
ACACATC450.00903847848.3333326
CTTATAC450.00903847848.3333321
Produced by FastQC (version 0.11.5)