FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_9424.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_9424.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25135995
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG36250.037.594814145
TCTCCGC117100.021.728432145
CACGAGC87700.020.581541145
AGACGGC151300.019.691603145
CTCCGAC38700.017.982018145
TATACTG324800.016.6955575
TACACTG219950.015.3924675
CTATACT190050.014.2283784
GTATAGG98700.014.0294341
ATGCCGG186100.013.711167145
GTGTAGG112550.013.5912951
TCTATAC183850.013.5646713
GTATAGA185250.013.5407221
ATACTGT408350.013.4038546
GTATTAG183850.012.9734721
GGATCGT39000.012.8251818
GTGTAAG167100.012.7988071
CTAGGAC113650.012.7577763
TAGACAG369600.012.690765
CTACACT166850.012.6873544
Produced by FastQC (version 0.11.5)