FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_7920.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_7920.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24023114
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG29950.034.610775145
TCTCCGC87150.017.633583145
TATACTG314850.016.647895
CACGAGC73150.015.161747145
GTATAGG92500.015.1268431
TACACTG222750.014.8087445
CTATACT192950.014.6160284
CTCCGAC31300.014.358844145
GTATAGA188150.014.06440451
TCTATAC180150.013.9643193
CTACACT163400.013.84294
CGAGACG20950.013.840384145
GTGTAGA156550.013.7542241
CTGTCGC82300.013.7403519
TACACCG26200.013.5587415
TACACAG272400.013.5467545
GTATTAG172400.013.4989651
ATACTGT400750.013.4051036
CCTACAC114350.013.1871743
GTGTAGG117800.013.0473791
Produced by FastQC (version 0.11.5)