FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_7784.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_7784.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29311470
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC229400.020.53955145
CTCCGAC63750.018.875519145
CACGAGC185100.018.797747145
TACACCG37850.016.855025
TATACTG331100.015.1297355
TACACTG247650.014.4318185
GTATAGG113400.013.8091131
CTACACT177050.013.1028724
GTGTAGG129800.012.9580111
GAGCCCC301200.012.947869145
CCCACGC233250.012.897259145
TAGTACT159100.012.6673894
GTAGGAC84300.012.6416053
GTATAGA212100.012.5102361
CCTACAC126200.012.465593
CTTACAC188350.012.3937493
GACTCCG119950.012.3886687
CTATACT210600.012.358014
CGAGACG58150.012.341173145
GTATTAG207250.012.2782831