FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_6119.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_6119.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23917790
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC122000.025.014795145
TCTCCGC153950.019.21123145
ATGCCGG186450.019.128345145
CTCCGAC66200.016.206095145
CACGAGC168700.015.984629145
TACACCG53600.014.7428625
TATACTG251700.014.5454865
TACACTG190200.013.9886445
GTATAGG90150.013.7520011
CTATACT164950.013.4050244
CGAGGCG176450.013.228448145
TCTATAC162650.013.1042853
GTATAGA161850.013.0799371
CTACACT143550.012.9792624
GTATTAG169900.012.929591
TAGACAG277400.012.6490815
CTAGTAC72400.012.6168823
TCTACAC148300.012.563543
GAGCCCC217000.012.526974145
GTGTAAG141900.012.5175461
Produced by FastQC (version 0.11.5)