FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_4340.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_4340.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24941303
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC137950.018.496723145
CACGAGC101550.016.48944145
TATACTG280000.015.6646565
TATACCG35050.015.5130345
TACACTG202000.014.8584145
GTATAGG104600.013.3771
CCTACAC110500.013.2529513
CTATACT184300.013.0597924
CTCCGAC52350.012.600813145
CCTATAC103250.012.5685873
TACACCG42450.012.4671935
CTACACT151950.012.3572714
GTAGGAC81800.012.319273
TCTATAC178400.012.27257253
GTGTAAG157200.012.2676731
GTATAGA179000.012.2317051
TACACAG250950.012.2201545
GTCCTAG101850.012.17217351
GTGTAGA164900.012.0905241
ATACTGT372050.012.0423356
Produced by FastQC (version 0.11.5)