FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_4329.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_4329.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26091313
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC144350.017.37543145
CACGAGC119850.016.633009145
TACACTG214400.016.095615
TATACTG300250.014.6565245
CTCCGAC59600.013.9871025145
CTACACT163550.013.3426244
TATACCG35550.013.25558955
CTTACAC162500.012.9826973
CTATACT194650.012.7378754
GTATAGA197750.012.6851771
CTAGGAC114500.012.6633843
GTATAGG104050.012.6116941
TACACAG260850.012.5068225
TAGACTG169250.012.4649225
GTATAAG174050.012.4130691
GTGTAGA172150.012.2552711
CCTACAC120300.012.1733773
TAGGACT156300.012.15255454
TCTATAC189300.012.1404273
ATACTGT387850.012.1312686
Produced by FastQC (version 0.11.5)