FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30982.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30982.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25740077
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG232650.016.0485695
TCTCCGC218000.016.027391145
GAGCCCC218500.015.061793145
TACACCG108200.014.271995
TACACTG154000.013.69949155
GTGTAGG136800.013.46167851
CTATACT177550.013.3523974
GTATAGG120350.013.3136691
GTATTAG191850.013.2268921
CTTACAC138250.013.1625723
TAGGACT130000.013.1613634
TATACCG71100.013.15383155
CCTACAC131900.012.8618443
GTATAGA193400.012.8584681
TCTATAC186950.012.7198093
TATACAG224300.012.6380395
GTGTAGA179800.012.5810381
CTAGGAC75100.012.3566913
CTACACT134600.012.3345314
CTACACC197950.012.3059734