FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30981.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30981.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24896274
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG67400.020.649939145
TCTCCGC115950.018.192806145
CACGAGC137200.017.59412145
ATGCCGG120450.016.550201145
TATACTG292050.016.184925
TACATCG90600.015.842162145
CTCCGAC41050.015.363266145
TACACTG218450.014.9009675
GTATAGA181750.014.3603141
GTGTAAG169650.014.1879651
TACACCG36300.013.9801435
GTATTAG184450.013.7177431
GTACTAG76700.013.6114071
GTGTAGG116050.013.4941181
CTATACT177700.013.0959474
CTACACT166050.013.0105814
CTAGGAC112650.012.9998843
GTATAGG98050.012.8658221
TCTATAC181900.012.6740013
TCTACAC157750.012.5921683
Produced by FastQC (version 0.11.5)