FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30979.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30979.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22169888
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG245000.016.807765
ATGCCGG63450.016.565748145
TACACTG198350.016.5209055
TCTCCGC80200.016.269447145
CACGAGC61550.016.01716145
CCTATAC87000.015.4162973
CTATACT163800.014.7386564
GTGTAGG102350.014.5213941
CTACACT143900.014.4593484
GTATAGG91500.014.1039631
GTGTAGA141350.014.0026131
TATACCG24950.013.9475615
ATACTGT315750.013.4320136
GTATAGA165700.013.2137691
TACACCG28050.013.1815015
ATGATCG60200.013.125163145
TAGACTG154150.013.0746145
CTAGGAC97550.013.0058383
TATACAG216950.012.7987245
TACACAG242650.012.7279045
Produced by FastQC (version 0.11.5)