FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30976.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30976.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28934856
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG83150.024.061102145
TCTCCGC133350.019.623789145
ATGCCGG149100.017.502237145
TACATCG111250.017.20175145
CACGAGC157250.017.148296145
TATACTG358000.015.3295825
CTCCGAC46500.014.809464145
TACACTG262400.014.3390715
CTATACT210750.014.3101184
TATACCG28900.013.7969255
TCTATAC215000.013.5551733
GTATAGG115400.013.4441381
GTATAGA214650.013.2735361
TACACCG38100.013.1293015
GTGTAAG187750.013.0515381
GTGTAGG134250.012.9605061
CTACACT183850.012.7366834
GTACTAG94600.012.4916961
CCTACAC135350.012.2657663
GTGTACG33700.012.2622441
Produced by FastQC (version 0.11.5)