FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30908.TR4_batch_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30908.TR4_batch_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19417240
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG114800.027.530722145
TCTCCGC124000.019.349926145
ATGATCG91150.019.245611145
TATACTG240600.016.3612865
CACGAGC100800.016.252527145
CTCCGAC40850.015.79323145
TGCCGTG100650.015.700582145
CTATACT140900.015.5385044
TACACTG163200.015.4586785
CGTCTTG99400.015.314609145
CGAGACA67100.014.908349145
TCTATAC140000.014.2920433
GTATAGA143850.014.0105411
CTACACT117700.013.85864
ATACTGT316650.013.5536256
GTATTAG132850.013.4789281
GTGTAGG87750.013.1362071
CCTATAC72550.012.7904493
TAGACTG132250.012.7724295
TATACAG189100.012.6896525
Produced by FastQC (version 0.11.5)