FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30893.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30893.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33111353
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG142800.044.519367145
CTCCGAC74800.021.126741145
TCTCCGC277450.020.90176145
CACGAGC335000.017.895271145
TATACTG379200.016.2700185
TACACTG268150.016.167775
CTACACT193850.015.8946124
GAGCCCC371000.014.966921145
CTATACT230850.014.9173114
TCTACAC200900.013.78510953
GTATAGG127050.013.63828851
GTATAGA227200.013.4979771
CCCACGC291050.013.324894145
CCTACAC139850.013.2710193
TAGACTG219650.013.2354885
CCACGAC108500.013.228575145
GCTACAC124250.013.1867833
GTGTAGG145200.013.1318451
TAGACAG412550.013.0744425
CTTACAC202550.013.0643393
Produced by FastQC (version 0.11.5)