FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30890.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30890.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25825855
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC116900.019.471113145
CACGAGC88600.016.772423145
TATACTG302150.016.2438135
TACACTG232600.015.3036045
GTGTAGG114800.014.3356871
GTATTAG187700.013.9436821
CTATACT199750.013.5739654
GTATAGA186850.013.5027011
TACACAG290700.013.4919325
GTATAGG102100.013.27854251
CGAGACG30700.013.222857145
TCTATAC186250.012.9230113
CTACACT162750.012.9181084
ATACTGT393300.012.7925846
TACACCG26650.012.7856435
CTCCGAC38750.012.720755145
TAGACTG181800.012.4417845
CTAGGAC113100.012.3713363
GCGAATG144550.012.286371145
TAGGACA171750.012.2411774
Produced by FastQC (version 0.11.5)