FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30849.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30849.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25525794
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC132300.019.231676145
CACGAGC98800.017.82868145
TATACTG314500.015.9284085
GTATAGG101350.014.4494131
CTCCGAC49150.014.158511145
TACACCG35250.013.5737495
GTATAGA185600.013.5151461
CTATACT195050.013.4919794
TACACTG205450.013.4441645
CTACACT161200.013.3569214
GTATTAG197200.012.7936641
TACACAG257350.012.7611265
ATACTGT402100.012.6746576
TAGTACT143250.012.55081754
GTGTAGG111900.012.3744511
TCTATAC185500.012.3106683
CCTACAC108450.012.1662523
CCTATAC98400.012.0826933
GTATAAG176650.011.9426851
CTAATAC208200.011.7693083
Produced by FastQC (version 0.11.5)