FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30844.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30844.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25911395
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC200200.016.112791145
TCTCCGC244300.015.459273145
TATACTG216150.015.1598215
TACACTG156250.013.8727345
TATACCG88800.013.7969985
TAGACAG175250.013.0719375
TACACCG119300.012.8826815
CTATACT187050.012.8674134
TACACAG178300.012.8483285
TAGACCG60900.012.7372995
TCTACAC187350.012.4211613
GTATAAG210100.012.3876611
CTAGGAC65550.012.276123
CTACACT134400.012.0825794
CTAGCCT102100.012.0707444
GTATTAG211000.012.0255931
CCTACAC138500.011.9866153
GTGTAAG148850.011.9326851
ACTGTCC111950.011.9143238
TAGACTG119350.011.9054145
Produced by FastQC (version 0.11.5)