FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30830.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30830.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27809040
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG171750.027.81389145
TAGATCG126300.022.6708145
TCTCCGC175350.021.207266145
CTCCGAC57450.020.062273145
CACGAGC249350.018.343958145
TGCCGTG137350.017.68028145
AGACCGG102700.016.657684145
CGTCTTG149100.016.23835145
TATACTG305100.016.2056925
TACACTG234700.014.4254435
GAGCCCC246350.014.153557145
CCACGAC78900.014.056832145
CCGTACG44600.013.977729145
CTATACT192950.013.6767214
TACACCG32950.013.6414855
GTGTAGG122200.013.4082951
CTTACAC177300.013.2483453
TACACAG287000.013.0092045
CTACACT172250.012.9212494
GTATAGG105700.012.8949361
Produced by FastQC (version 0.11.5)