FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30786.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30786.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26513779
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC146650.020.90903145
CACGAGC100200.017.869188145
TATACTG311150.015.0050255
TACACTG223800.014.7715275
CTCCGAC50850.013.827904145
GTATTAG196300.013.2589121
CTAGGAC111550.013.1931073
GTGTAGG116450.012.9496361
GTATAGA195300.012.7328491
CTACACT164600.012.7288354
TAGGACT160050.012.6830284
GTATAGG106200.012.5610821
GTATACG32050.012.4413761
CTATACT189100.012.4215024
CCTACAC115950.012.3798413
GTAGGAC83150.012.2063673
TAGTACT144850.012.0119464
GTCTAAG124600.011.98623851
TCTATAC190350.011.8828973
GTACTAG83900.011.7519711