FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n01_MG_30782.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30782.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28227174
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG125100.020.628178145
TACACCG129650.015.6006035
TATACTG235200.015.503885
GAGCCCC201100.015.103239145
TCTCCGC230050.014.904144145
TACACTG158800.014.8368795
GTATAGG134500.014.2299071
TATACAG244700.013.3021565
CTACACT145650.013.2895564
GTATAGA223200.013.0897491
GTGTAGG144550.012.8894831
CTATACT200600.012.86556154
CCTACAC146400.012.627253
GTATTAG221450.012.5057041
TCTATAC218500.012.3756233
TAGACAG190750.012.351755
CCTATAC135250.012.3282173
TACACAG194350.012.1975575
CTAGTAC80900.012.1870923
TAGACTG126400.012.0443095
Produced by FastQC (version 0.11.5)