FastQCFastQC Report
Fri 16 Aug 2019
H2HMJBGXC_n01_MG_30782.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n01_MG_30782.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25571165
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG81500.027.0385145
TCTCCGC167250.017.033075145
TACACCG70550.015.2081495
CACGAGC184450.015.169641145
TATACTG259650.014.5465845
GTATAGG103350.014.3802381
TACACTG191700.013.8411025
TATACCG47450.013.59775
CTAGTAC79300.013.4386293
GTGTAGG120450.013.0609751
GAGCCCC212900.012.836078145
CTATACT179500.012.6816584
GTATTAG195750.012.5551131
GTATAAG170200.012.5230611
GTGTAAG150100.012.3646551
TAGACAG281050.012.3040045
TACACAG230350.012.1481675
GTCTAAG114850.012.119731
GTATAGA185050.012.0665841
CCTACAC122000.011.9439243
Produced by FastQC (version 0.11.5)