FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15638648
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6505634.159969583048356No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC750350.4798049038510234Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA157600.048.16842316
AGAGCAC163500.047.5650715
GCACACG89150.047.385318
ACACGAG52400.045.55220820
CACACGA54550.043.8854419
AGCACAC110300.043.88395717
CACGAGA59000.040.09984221
CACGTAG44800.036.17020821
AAGAGCA281900.034.2792914
GCACAAG76300.032.3386818
CACACGT58350.030.95052319
ACACGTA52900.030.50012420
CAGATCG179600.030.2864426
ACGAGAT79900.029.21623622
CGAGATC84550.028.47869523
TAGATCG195800.028.2275376
AAAGGGG140600.026.6848366
AGCACAA98100.025.54483617
GCAGATC78650.024.567875
AAGGGGG153400.024.29887467
Produced by FastQC (version 0.11.2)