FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19483747
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6394563.2819970409182586No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1250370.6417502752422314Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG64150.046.81077220
AGAGCAC209300.046.5707615
GAGCACA205350.046.39282616
GCACACG121100.045.40339718
CACACGA66800.044.90147419
AGCACAC145350.041.65704317
CACGAGA74050.040.83553321
AAGAGCA359250.034.0590114
CACGTAG56500.033.88351421
AAAGGGG187650.033.19869666
GCACAAG97100.032.5840818
CACACGT82850.028.38788419
AAGGGGG223200.027.73894367
ACGAGAT107550.027.72567222
ACACGTA69350.027.05038620
GGGGGAT49950.026.2418651
CGAGATC117550.025.75403823
CAGATCG268400.025.1427066
AGCACAA130550.024.61065517
CCGTATC419000.023.57154849
Produced by FastQC (version 0.11.2)