FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_rad27-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_rad27-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654977
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3882832.843527308760754No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1414711.036039826357818Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC199200.052.63919415
GAGCACA196400.051.9460216
ACACGAG63500.051.53390520
CACACGA63300.051.30969619
GCACACG115850.049.99815418
AGCACAC138050.048.2457617
CACGAGA67500.047.8056421
CACGTAG48850.044.06183221
AAGAGCA324550.040.15903514
GCACAAG83100.038.74706318
AAAGGGG195250.038.0549366
ACACGTA56800.037.2172120
ACGAGAT89100.035.70575322
CACACGT70350.035.0735919
AAGGGGG220750.033.5960867
CGAGATC102700.032.13610523
AGCACAA101650.031.6420717
CAGATCG229000.029.9280576
CGTAGAT73250.029.76681923
GCAGATC90300.029.7316135
Produced by FastQC (version 0.11.2)