FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14408597
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3673282.549366881452788No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC710450.49307368371813026Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103200.049.95624518
GAGCACA172200.049.93852216
AGAGCAC178100.049.5615515
CACACGA63300.047.88311819
ACACGAG63750.047.65409520
AGCACAC125250.046.47075317
CACGAGA71600.042.477321
CACGTAG47450.037.9120321
AAGAGCA298350.035.96746414
GCACAAG75850.032.9003618
CACACGT63250.032.8142419
ACGAGAT92350.032.74409522
ACACGTA54350.032.45575320
AAAGGGG126650.028.29768466
CGAGATC110850.027.87915823
CAGATCG199400.026.191036
CGTAGAT70850.026.08247223
AAGGGGG139650.025.7140367
AGCACAA100000.025.16483517
ACGTAGA78600.023.95608722
Produced by FastQC (version 0.11.2)