FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12783508
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4617433.612021050872734No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1113350.8709268222775783Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG97250.059.0576620
CACACGA98200.058.45114519
AGAGCAC321250.057.8075815
GAGCACA314600.057.53875716
CACGAGA100750.055.9627821
GCACACG174150.054.60429818
AGCACAC207900.052.84401317
GCACAAG122100.051.9974818
AAGAGCA470150.049.26622814
CACGTAG67100.048.6640121
AGCACAA138200.046.14230317
ACGAGAT122450.045.5874822
ACACGTA71550.044.75779720
CACAAGA148950.042.74180219
GCAGATC112500.040.5429575
GAGCAAG95750.040.35440416
CGAGATC145150.040.04942323
CACACGT91000.039.69163519
CAGATCG303350.039.5434846
CACACAG39950.039.33600219
Produced by FastQC (version 0.11.2)