FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_exoi-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_exoi-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215107
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8468437.550913245856683No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2519922.2468978673141504Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA134000.060.8301719
ACACGAG135050.060.14909420
AGAGCAC434700.059.459215
GAGCACA426250.059.34086216
GCACACG248300.057.79091318
AGCACAC288400.056.56425517
CACGAGA144000.056.11875521
CACGTAG101350.056.11464321
AAGAGCA574350.055.8061714
GCAGATC212750.054.278415
ACACGTA102100.053.7488120
GCACAAG153800.053.24905818
CAGATCG628650.051.263946
CCAGATC156200.049.6595885
AGCACAA168600.048.76224517
ACGAGAT167750.047.58930222
CACACGT126950.047.14305519
CTAGATC156650.046.2545365
GGCAGAT94850.046.0955354
CACAAGA183850.044.4886719
Produced by FastQC (version 0.11.2)