FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12571625
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5621854.471856263609517No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC982320.7813787000487208Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA78850.056.06200819
ACACGAG79300.055.83103620
GCACACG136900.055.3249218
AGAGCAC232200.055.3028315
GAGCACA228150.055.28761716
AGCACAC163650.051.4142817
CACGAGA85350.051.3393721
CACGTAG57850.047.55201321
AAGAGCA346150.044.50896514
GCACAAG88900.042.20462418
ACACGTA64050.041.74770420
ACGAGAT106000.040.87563722
CACACGT75650.040.11226319
CGAGATC119750.036.97115323
AGCACAA103400.036.31988517
CAGATCG242750.035.6730546
GCAGATC96750.034.116875
AAAGGGG154300.033.547466
CGTAGAT83200.033.31577323
TAGATCG267450.032.2083786
Produced by FastQC (version 0.11.2)