FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_dna2-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_dna2-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17239687
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7325554.249236079518149No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC898560.5212159594312821Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG68550.044.11342618
AGAGCAC135250.042.98338715
GAGCACA132700.042.72810716
ACACGAG43550.037.36988420
CACACGA43150.037.311419
AGCACAC92600.036.17183317
CACGAGA51150.031.67996821
CACGTAG40250.029.65090221
AAAGGGG154450.029.54892266
AAGAGCA275150.027.59034314
GCACAAG76700.026.1014818
CACACGT51550.025.8678819
AAGGGGG180900.025.34550967
ACACGTA48700.022.99735820
CCGTATC309350.022.54733749
TAGATCG216600.022.3983426
CAGATCG207350.021.9963956
CGCCGTA325600.021.3361247
GCCGTAT332600.021.24477448
CGTATCA333750.021.03537450
Produced by FastQC (version 0.11.2)