FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_dna2-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_dna2-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482447
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8753496.49250837032773No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5603844.156396832118086Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA412650.065.3855819
ACACGAG414050.065.20531520
AGAGCAC1286600.063.08135615
GAGCACA1257250.063.06184416
CACGAGA428450.062.51572821
AAGAGCA1590600.062.22313714
CACGTAG286800.062.09057621
GCACAAG392500.061.83975618
ACACGTA279400.061.830820
AGCACAC852250.061.4117317
GCACACG770750.061.266618
AGCACAA402950.060.27076317
GCAGATC518300.059.796275
GGCAGAT197400.059.1405534
GAGCAAG270200.058.3282816
GCACGCG42400.058.27734818
CCAGATC382150.057.788635
GCCAGAT145050.057.3892824
GCACACA100800.056.76990518
ACGAGAT467700.056.5432422
Produced by FastQC (version 0.11.2)