FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n02_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n02_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171521
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4456663.3835576012823423No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC413150.31366916546691914Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG59300.047.57027418
AGAGCAC108000.045.95235415
GAGCACA106450.045.6351116
CACACGA37050.043.92566719
ACACGAG36700.043.77189320
AGCACAC72950.043.6589217
CACGAGA44250.036.06646321
CACGTAG31950.034.61530721
CACACGT40500.029.3817419
AAGAGCA215000.028.8943914
ACACGTA38650.027.34677720
GCACAAG57450.026.62236818
CGAGATC60600.026.56657223
CAGATCG132650.026.4921996
ACGAGAT63200.025.08622
AAAGGGG92100.024.0541266
TAGATCG134700.023.6464186
GATCGGA507650.021.780788
CGGGGAC11550.021.5446191
AGATCGG512950.021.5216187
Produced by FastQC (version 0.11.2)