FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15638648
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG740710.4736406881208657TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG74800.029.1207311
CGTGTGC110750.025.5178031
ATGCCGG26850.024.50571370
CTGCTTG256350.022.9231358
GTGTGCT132600.021.280412
TGCTTGA279550.021.12095359
GCTTGAA297400.020.0416360
CCGTCTT308300.019.03758851
GCGCGGG35950.018.98407670
TGCCGTC320150.018.45318449
CCCCGGG39850.018.355917
GCCGTCT320150.018.34386450
CGCGCGG38050.017.8444769
CCCGGGT41600.017.8361238
TCTGCTT333700.017.74589757
CTTCTGC349900.017.56444255
TGCCCCG41850.017.4838835
GACGTGT45450.017.2594761
CGTCTTC348500.017.07255652
ATGCCGT348500.016.91187348
Produced by FastQC (version 0.11.2)