FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19483747
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG971130.49843082031397756TruSeq Adapter, Index 20 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG311500.026.88660858
TGCTTGA344650.024.42245759
ACGTGTG126450.024.1781621
GCTTGAA375600.023.40710360
GACGTGT84200.022.8343241
CGTGTGC180350.022.5252551
ATGCCGG60350.021.86339870
GCCGTCT371900.021.60718550
TGCCGTC401850.021.52959449
CCGTCTT376750.021.50553351
TCTGCTT405050.020.9706857
GTGTGCT202700.020.2608832
ATGCCGT432900.020.26025448
CTTCTGC400600.020.07666655
CGTCTTC407050.019.79292952
TATGCCG464750.019.44416647
CTTGAAA470500.019.11732361
TTCTGCT420650.018.91999256
GTCTTCT429600.018.44440553
AAAAAGG377700.018.29168970
Produced by FastQC (version 0.11.2)