FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_rad27-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_rad27-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654977
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG1366491.0007266947428766TruSeq Adapter, Index 15 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG31250.032.3669970
ACGTGTG98000.031.7650591
CTGCTTG349000.031.37890458
TGCTTGA361550.030.38649459
GCTTGAA372150.029.81264360
CGTGTGC154000.027.5130421
TCTGCTT417650.026.33850557
AAAAAGG304300.026.14279270
CTTGAAA430350.026.09789861
CTTCTGC422200.025.91382855
CCGTCTT435650.024.95291951
TTCTGCT447450.024.3809156
GTCTTCT451900.024.15631353
TCTTCTG452550.024.09068354
GCCGTCT451750.024.07136550
GTGTGCT177550.024.0360532
TGCCGTC462600.023.88498749
CGTCTTC461900.023.6484952
GACGTGT55650.022.9668621
ATGCCGT496800.022.28299948
Produced by FastQC (version 0.11.2)