FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14408597
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG613550.42582216714090904TruSeq Adapter, Index 14 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG36050.030.29028370
ACGTGTG93550.029.1596851
CGTGTGC145150.027.333931
GACGTGT55250.024.2748011
GTGTGCT166700.023.8589862
CTGCTTG212500.023.66755358
TGCTTGA234050.021.69780559
GCTTGAA250700.020.78726660
TGTGCTC211200.019.01413
GCCGTCT261950.018.79864950
CCGTCTT262950.018.7271651
TGCCGTC279450.018.54820649
GTGCTCT219200.018.4159244
TCTGCTT286550.017.93003757
CTTCTGC283700.017.54255155
ATGCCGT300800.017.30151648
CTTGAAA314400.016.9763561
CGTCTTC296400.016.731852
AAAAAGG238950.016.40457570
GTCTTCT301300.016.38999753
Produced by FastQC (version 0.11.2)