FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12783508
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG843070.659498159660087TruSeq Adapter, Index 10 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG142550.037.417021
GACGTGT65450.032.158911
CGTGTGC260700.030.7497251
ATGCCGG94150.028.58623970
GTGTGCT289200.027.748462
CTGCTTG284000.022.82313764
AAAAAGG264500.022.15036270
TGCTTGA305000.021.74513465
GCTTGAA330350.020.97692766
TGTGCTC389200.020.68193
GTGCTCT401200.020.0545734
CTTCTGC349300.019.4682561
TCTGCTT357250.018.53538563
CTTGAAA387750.018.38605567
CCGTCTT374250.017.88967757
GTCTTCT368050.017.53497359
TTCTGCT362100.017.50421562
TCTTCTG370600.017.37661460
CGTCTTC381150.017.17097758
CGGCGTG16150.017.12007170
Produced by FastQC (version 0.11.2)