FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_exoi-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_exoi-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215107
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG2275092.0285941097129077TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG192450.043.2504581
CTTGAAA1344100.042.62053367
GCTTGAA1342050.042.34158366
TGAAAAA1442150.041.70070369
TGCTTGA1329900.041.68381565
GAAAAAA1457500.041.67934870
CTGCTTG1332650.041.6319464
TTGAAAA1405950.041.04928668
CTTCTGC1394400.040.27272861
TCTGCTT1383450.040.25754563
TTCTGCT1372050.039.8777962
GTCTTCT1425300.038.92307359
TCTTCTG1434500.038.6931460
AAAAAGG357650.038.54628870
CCGTCTT1530850.037.06896257
CGTCTTC1530650.036.93220558
GCCGTCT1568550.035.99281356
TGCCGTC1646300.034.96917355
ATGCCGG104400.034.32845770
ATGCCGT1720950.033.5600954
Produced by FastQC (version 0.11.2)